FastQCFastQC Report
Wed 25 May 2016
SRR1033276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTT130.5471380471380471No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTC130.5471380471380471No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGC130.5471380471380471No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCAC100.42087542087542085No Hit
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTT80.33670033670033667No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG70.2946127946127946No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGAC70.2946127946127946No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCC70.2946127946127946No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGC70.2946127946127946No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCC60.25252525252525254No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAAT60.25252525252525254No Hit
CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGC60.25252525252525254No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA60.25252525252525254No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGA60.25252525252525254No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGA60.25252525252525254No Hit
CTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTA50.21043771043771042No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCT50.21043771043771042No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGG50.21043771043771042No Hit
GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCG50.21043771043771042No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGC50.21043771043771042No Hit
CTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTC50.21043771043771042No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGA50.21043771043771042No Hit
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGC40.16835016835016833No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCG40.16835016835016833No Hit
GCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCAC40.16835016835016833No Hit
ATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTC40.16835016835016833No Hit
TAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGA40.16835016835016833No Hit
ATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTA40.16835016835016833No Hit
CCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGG40.16835016835016833No Hit
TCTCTGTACATCCCATCAAGGGTACATACTGTAGGTTTGTCTGTCACTGATGATGTTAAATTTGATCACTTTGTT30.12626262626262627No Hit
GCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGT30.12626262626262627No Hit
CGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACC30.12626262626262627No Hit
CGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTAT30.12626262626262627No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAG30.12626262626262627No Hit
GCTGTGGGAGCATCCACAGTCGAGCGGGGGTTCTTGATGGTTTCATCTACAGACACGATCAGGCACGCAGCCTCA30.12626262626262627No Hit
GTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTC30.12626262626262627No Hit
GAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGG30.12626262626262627No Hit
GAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTT30.12626262626262627No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGC30.12626262626262627No Hit
GAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAG30.12626262626262627No Hit
CCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCT30.12626262626262627No Hit
ACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGT30.12626262626262627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCA150.002073052869.021
CATCTCG150.002073052869.026
GAAAAAA206.871419E-569.050
ACTCCTG150.002073052869.017
CCTGAGC150.002073052869.020
GAGCATC150.002073052869.023
TGCTTGA206.871419E-569.045
GCTTGAA206.871419E-569.046
AGCATCT150.002073052869.024
CTGCTTG206.871419E-569.044
TTGAAAA206.871419E-569.048
CGAGACT150.002073052869.013
AGACTCC150.002073052869.015
GACTCCT150.002073052869.016
TGAGCAT150.002073052869.022
GAGACTC150.002073052869.014
TGAAAAA206.871419E-569.049
GTATGCC252.0744855E-455.232
ATCTCGT252.0744855E-455.227
GCCCACG252.0744855E-455.28
Produced by FastQC (version 0.11.5)