FastQCFastQC Report
Wed 25 May 2016
SRR1033275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860024
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112250.3924792239505682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.033.9365351
GTATCAA83100.026.8344481
ATCAACG103750.021.9804883
TCAACGC104600.021.7643954
CGCGTAT800.002952470221.49656312
CAACGCA106600.021.3241715
TATCAAC105050.020.9595282
AACGCAG110400.020.7466686
GTGGTAT25400.019.9965361
ACGCAGA125900.018.1111587
GAGTACT81850.017.90110812
CGCAGAG127550.017.8768738
AGAGTAC119500.017.84484711
TGGTATC26950.017.6821732
GTACATG78800.017.4825441
AGTACTT84400.017.23830413
GTACTTT88600.016.50458714
GCAGAGT135800.016.4880879
CAGAGTA133000.016.47315610
TACATGG78400.016.4600722
Produced by FastQC (version 0.11.5)