FastQCFastQC Report
Wed 25 May 2016
SRR1033275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860024
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.034.2679181
GTATCAA79600.027.8724171
ATCAACG99350.022.0722263
TCAACGC100250.021.9772534
TATACCG1605.7625584E-821.5494885
CAACGCA103150.021.3259495
TATCAAC103750.021.235852
AACGCAG106350.020.7822536
GTGGTAT25550.018.3950371
ACGCAGA122100.017.9602997
GAGTACT81850.017.8194312
AGAGTAC118350.017.80102511
CGCAGAG123800.017.713678
GTACATG75800.017.59831
TGGTATC25800.017.506872
AGTACTT85100.017.13829813
CGTATAC1452.7367685E-416.645123
TACATGG77700.016.5074062
GCAGAGT131500.016.4928999
CAGAGTA130150.016.37255110
Produced by FastQC (version 0.11.5)