FastQCFastQC Report
Wed 25 May 2016
SRR1033274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6050577
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA403530.6669281293337809No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN237360.3922931647675916No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA114430.1891224588993744No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA106130.17540475891803375No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG92060.15215077834725516No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC84790.14013539535155078No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC66970.1106836587651062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA1150.001105750918.01262765
GAGTACT107300.016.82960512
GTACATG191050.016.4522291
TACATGG198200.015.4536762
TATACGG2251.8934097E-615.4517272
CGACCGT2453.0121737E-715.44910819
AGAGTAC215850.015.44150611
AGTACTT117600.015.32684213
TATACCG3151.4224497E-915.285055
ACATGGG195900.015.0305653
GTACTTT128150.014.28382614
TATACTG21100.014.1803245
ATCCGAT3657.4578566E-1014.13578610
GTATATA17800.014.0642271
GTCGCGA2253.450136E-513.81175448
CGGTGTA1800.001436652813.37686413
TACTTTT142950.013.0462215
CATGGGG137050.012.8230474
TACGACC3053.5764606E-612.4034414
GTATACG3103.7954342E-612.3377041
Produced by FastQC (version 0.11.5)