FastQCFastQC Report
Wed 25 May 2016
SRR1033274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6050577
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA439430.7262613135904229No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA133990.22144995427708794No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA111660.18454438312246915No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG107180.17714013060242023No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC92580.15301020051476083No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA83840.1385652971609154No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC72400.11965800947579049No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG62080.10260178492067781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9350.020.65127443
CTCGTAT10450.018.14750139
CGCCGTA1602.9682675E-517.24012648
ATCTCGT10650.017.15918537
TATAACG2502.0550942E-816.550522
GAGTACT109400.016.48370412
GTACATG192550.016.1851671
TACATGG195650.015.7377282
TATACTG21200.015.6136995
AGTACTT116550.015.32455713
AGAGTAC215000.015.15527311
ACATGGG187500.015.1529213
TCTCGTA12550.014.83612338
GTACTTT127550.014.16515214
GTATAGA12050.013.7628491
GTATAAC12500.013.5437911
GACCGTA2304.299479E-513.492277
GATCGTA7950.013.44512821
TGCCGTC13950.013.34719545
GTATATA16200.013.2230251
Produced by FastQC (version 0.11.5)