FastQCFastQC Report
Wed 25 May 2016
SRR1033273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171847
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126680.39938874731347385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.040.1740231
GTATCAA86650.030.77631
GTGGTAT20550.028.085741
TGGTATC21350.026.216152
ATCAACG109150.024.803083
TCAACGC111350.024.3725034
CAACGCA113250.023.9332315
TATCAAC113700.023.3600522
AACGCAG117400.023.0289766
ACCGCGT1507.549843E-720.639498
ACGCAGA132250.020.3143587
CGCAGAG132700.020.1158588
AGAGTAC133250.018.7423511
GAGTACT97750.018.33477412
CAGAGTA139050.018.33166110
GCAGAGT141850.018.0428499
GTACATG81100.017.920291
AGTACTT101850.017.32705313
TACATGG80500.017.2744832
GTACTTT106550.016.56720514
Produced by FastQC (version 0.11.5)