FastQCFastQC Report
Wed 25 May 2016
SRR1033273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171847
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50200.042.9592131
GTATCAA90700.029.911471
GTGGTAT20200.025.6634651
TGGTATC21000.023.4803852
ATCAACG113250.023.4140513
TCAACGC113750.023.3111324
TATCAAC115800.023.047342
CAACGCA115350.022.9280035
AACGCAG119650.022.1616526
ACGCAGA138800.018.9798327
CGCAGAG139050.018.896118
AGAGTAC141100.018.08451511
ACCGCGT1351.5844716E-417.879398
GAGTACT100600.017.515612
CAGAGTA147500.017.43982310
GCAGAGT149850.017.2120789
CTGTCGC6250.017.1029159
GTACATG87050.017.031931
AGTACTT104150.016.88546613
ACGAACG1250.001948326816.55146829
Produced by FastQC (version 0.11.5)