FastQCFastQC Report
Wed 25 May 2016
SRR1033272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372819
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53660.39087454354871254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.1356333209257739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.11516449000195947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.028.9465751
ATTCGTT500.00782312527.536317
TATACCG500.00785114827.516135
GTATCAA91350.024.8172781
TACGCTC1202.4489673E-623.01016836
ACGACTT850.00410828620.30458367
AACGCAG116450.019.8189376
ATCAACG117700.019.7574653
TCAACGC117650.019.7337324
CAACGCA118150.019.5337735
TATCAAC115000.019.4392742
GTTCGGT900.00581202619.12242916
GAGTACT70050.019.05323212
AGTACTT72700.018.40603413
TATAGTG1702.5047302E-618.2092045
GGTCGCG950.00797386618.1027167
ATGCGTC1150.001106258818.00994354
CACGTAG1150.001106804718.0086263
GTACATG90850.017.796851
TACATGG89750.017.6643352
Produced by FastQC (version 0.11.5)