FastQCFastQC Report
Wed 25 May 2016
SRR1033272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372819
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.11683987473949588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.10008602736413176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG400.002603019134.4823928
GGTATCA62750.029.4551181
TATGCCG7750.029.36457643
CACTCCG500.007638267727.67158169
ATCTCGT8300.025.7569937
TCTCGTA8250.025.07718738
CTCGTAT8600.024.85849239
TGTACGA851.4215152E-424.40807557
ATGCCGT9250.024.22998444
TGCCGTC9700.023.10591545
GTATCAA92100.022.8443281
CCGTCTT10000.022.75754747
CGTATGC9850.022.75404741
CGTCTTC10400.021.55070948
GTTGCGC800.00290893421.55070932
TCGTATG10700.021.26873640
TACAGCG1154.6229143E-520.9885167
CTAGATC10000.020.6886833
CGCACAT1004.3514278E-420.6886834
ACTAGAT10400.020.5560632
Produced by FastQC (version 0.11.5)