FastQCFastQC Report
Wed 25 May 2016
SRR1033271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369396
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87910.37102282607044157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.036.426751
GTATCAA65500.028.7259331
TAACGCA1951.8189894E-1224.687264
TCAACGC86100.022.3647614
ATCAACG85750.022.2980143
CAACGCA87750.021.9838815
TATCAAC86700.021.4971052
AACGCAG90450.021.2529646
TATACCG1154.7236303E-520.9309445
TATAACG1901.4622856E-820.1313722
GTGGTAT18400.019.0906941
GTACATG72800.019.013841
ACGCAGA104600.018.2146997
TACATGG72200.018.1086032
CGCAGAG106050.017.8359368
AGAGTAC100300.017.38441811
TGGTATC20800.017.2190112
TATAGCG1200.001509063617.1954657
GAGTACT65950.017.1567212
ACACCGT3257.2759576E-1216.9298426
Produced by FastQC (version 0.11.5)