FastQCFastQC Report
Wed 25 May 2016
SRR1033271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369396
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.037.624981
GTATCAA63850.027.4818861
TCAACGC81500.021.321994
ATCAACG81350.021.3189223
CAACGCA83700.020.7203645
GTGGTAT17850.020.705671
TATCAAC84700.020.475732
AACGCAG87000.020.0533126
GTACATG75750.018.9694351
TGGTATC19350.018.7095412
TACATGG75000.018.021032
ACGCAGA102500.016.9199457
TAGGACC5550.016.7735754
ACATGGG78450.016.6571663
CGAACTA2702.9485818E-916.60100729
CCGCGCA1250.001949344316.5499279
CGCAGAG104200.016.5446328
GAGTACT67150.016.4821512
AGTACTT68000.016.27612113
AGAGTAC102750.015.9727611
Produced by FastQC (version 0.11.5)