FastQCFastQC Report
Wed 25 May 2016
SRR1033270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3007013
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN116650.3879264905073573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48500.038.9394841
GTATCAA80200.029.4027141
GTGGTAT20250.024.045571
ATCAACG103300.023.341863
TCAACGC103600.023.1711714
TATCAAC102450.022.708452
TATAACG1854.3837645E-1022.5570772
CAACGCA106950.022.3489115
AACGCAG110450.021.7971236
TGGTATC24050.019.2313562
TATGTCG6100.019.1690865
ACCGTCC2204.3582986E-918.760318
ACGCAGA127250.018.757857
CGCAGAG129000.018.4767238
GAGTACT79400.018.19507212
AGAGTAC128400.017.30583211
GTACATG92900.017.1467511
AGTACTT83850.017.10637513
GCAGAGT136450.016.9402859
CAGAGTA134500.016.77670110
Produced by FastQC (version 0.11.5)