FastQCFastQC Report
Wed 25 May 2016
SRR1033269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256421
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129320.3971230992552867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49600.040.3160171
GTATCAA88300.029.8144381
GTGGTAT20450.026.018941
ATCAACG109500.024.2785113
TCAACGC110400.023.9800974
CAACGCA113300.023.3059795
TATCAAC113050.022.6984352
AACGCAG117100.022.5793866
TGGTATC23350.021.2940982
ACGCAGA132550.019.922527
CGCAGAG133650.019.6302178
GAGTACT85300.018.70648212
AGAGTAC130100.018.52950711
GTACATG96450.017.9563711
GCAGAGT141750.017.9527849
CAGAGTA139200.017.88638110
AGTACTT89350.017.55120313
TACATGG95050.017.4858152
GTACTTT92850.016.8572514
ACATGGG100550.016.3836293
Produced by FastQC (version 0.11.5)