FastQCFastQC Report
Wed 25 May 2016
SRR1033269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256421
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54850.041.1309051
GTATCAA90400.027.6695351
ATCAACG111900.021.9087123
TCAACGC112200.021.788674
GTGGTAT23800.021.774391
CAACGCA115450.021.1753035
TGGTATC23300.021.1620372
TATCAAC115950.021.1434652
AACGCAG118700.020.7117186
ACGCAGA138800.017.5385067
GTACATG100600.017.514751
GAGTACT86850.017.15133512
CGCAGAG141900.017.1310528
TACATGG99450.016.7810022
AGAGTAC137100.016.49872611
ATACCGC1907.064191E-616.3330126
AGTACTT92250.016.18473213
GTACTTT93150.015.95432414
CAGAGTA147100.015.72849610
GCAGAGT151300.015.7248859
Produced by FastQC (version 0.11.5)