FastQCFastQC Report
Wed 25 May 2016
SRR1033268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205404
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86080.39031397421968944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.039.4678841
GTATCAA57950.027.6127871
GTGGTAT15800.025.9794981
ATACCGC1653.270543E-922.9236976
TGGTATC16600.022.6293142
ATCAACG72000.022.0655693
CGAACTA1906.675691E-1021.81068429
TCAACGC73050.021.6998464
TATCAAC73850.021.382632
CAACGCA74750.021.2523385
AACGCAG77700.020.4454616
CGTATAC1108.4222347E-418.7570343
GCGAACT2651.1459633E-1018.24415828
ACGCAGA88550.017.7477467
GTACATG60200.017.6819741
CGCAGAG89750.017.3571838
ACAACGC1804.33101E-617.1943428
TACATGG60000.016.6954042
GAGTACT58700.016.46166812
TACACTG11950.016.4014725
Produced by FastQC (version 0.11.5)