FastQCFastQC Report
Wed 25 May 2016
SRR1033268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205404
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.041.3920941
GTATCAA60150.027.0590271
CGCAACG658.6885947E-426.52328924
TAACGGC1057.7273944E-726.27068736
GTGGTAT16350.022.4034541
TGGTATC16550.021.2506832
ATCAACG77250.021.1121943
TCAACGC77500.020.9551094
CAACGCA79550.020.4150965
TATCAAC79900.020.4119782
TACACCG2051.7553248E-920.1835775
AACGCAG82250.019.912626
CGCGGTA1108.271739E-418.80742343
CGACGTT1150.001090900418.04822267
TTAACGG1753.2280677E-617.73271435
ACACCGT2952.910383E-1117.5323436
GTACATG62650.016.8230841
CGCAGAG97250.016.7703558
ACGCAGA97050.016.7693867
GACGTTA1452.6688047E-416.69979168
Produced by FastQC (version 0.11.5)