FastQCFastQC Report
Wed 25 May 2016
SRR1033267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394885
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55280.39630507174426566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.042.2585261
TTAACGG901.2187229E-1038.35964635
GTATCAA46900.032.080281
TAACGGC1101.0513759E-931.38516436
CGCGGTA1052.3072062E-829.60350643
CGGTACC1155.5819328E-827.0292945
AACGGCC1306.2609615E-926.55763837
TATAACG1057.222916E-726.473292
ACAACGC1306.537448E-926.450453
GTACCTG9650.026.2856431
TCTGTCG4600.026.1601188
ATCAACG58450.025.9436153
TCAACGC59350.025.4895084
TACCTGG9450.025.3702372
CAACGCA60750.024.9586895
TATCAAC59950.024.7483332
GTGGTAT9600.024.2508321
CTAACCG1301.8312494E-723.90706452
TAACCGT1301.8312494E-723.90706453
AACGCAG63900.023.8359436
Produced by FastQC (version 0.11.5)