FastQCFastQC Report
Wed 25 May 2016
SRR1033267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394885
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.038.7086561
GTGGTAT10700.030.9910431
CGCTAGC450.004633590630.65235947
GTATCAA45450.029.4880871
TACCTGG9750.027.9408052
GTACCTG10500.027.6336821
ATAACGC1503.45608E-1127.5871263
TGGTATC12000.027.5871262
TATGCCG500.007753110527.58712443
TCTGTCG4700.026.4132028
GTACCGG1456.9303496E-1026.2043511
ATCAACG57100.023.1301863
TATCAAC57700.023.0689542
TCAACGC58400.022.7333974
CAACGCA59550.022.2364715
AACGCAG62300.021.4763346
GTCTCAC6450.021.4214571
TAGACTG2900.021.4038035
TATAACG1808.631105E-921.0734962
GTATAAC3000.020.725261
Produced by FastQC (version 0.11.5)