FastQCFastQC Report
Wed 25 May 2016
SRR1033266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469150
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN175330.3923117371312218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.038.725651
GTATCAA94000.029.1046641
TCAACGC118750.023.2239344
ATCAACG118750.023.1409663
TGGTATC27450.022.5394842
GTGGTAT28900.022.4936261
CAACGCA123400.022.3209345
AACGCAG127650.021.6054466
TATCAAC126100.021.3349782
GACGCGT1004.4321734E-420.63386210
ACGCGTT1004.4321734E-420.63386211
GTACATG114650.019.7388881
TACATGG114250.019.014732
ACGCAGA145950.018.7323517
CGCAGAG147750.018.4575888
GAGTACT100000.017.95145812
ACATGGG122350.017.5688923
AGAGTAC149150.017.03924811
AGTACTT104800.016.83430313
CAGAGTA156900.016.41678610
Produced by FastQC (version 0.11.5)