FastQCFastQC Report
Wed 25 May 2016
SRR1033266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469150
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.039.2821461
GTATCAA102700.027.8520831
ATCAACG127850.022.196513
TCAACGC128650.022.0852854
CAACGCA132300.021.4759795
GTGGTAT28700.021.3053741
TATCAAC135950.020.9754812
AACGCAG137350.020.7365726
GTACATG123300.018.7999731
TGGTATC30500.018.7669562
TACATGG123950.018.249162
ACGCGTT1150.001114836717.99052611
ACGCAGA160050.017.6662257
CGCAGAG161750.017.395288
ACATGGG127150.016.976323
GAGTACT105250.016.38092212
AGAGTAC162250.016.19424611
CAGAGTA170400.015.7635310
GCAGAGT175050.015.6993549
AGTACTT110650.015.42567813
Produced by FastQC (version 0.11.5)