FastQCFastQC Report
Wed 25 May 2016
SRR1033265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3579
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTT150.4191114836546521No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTC140.39117071807767534No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAG130.3632299525006985No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAAT100.2794076557697681No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCAC100.2794076557697681No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGA90.2514668901927913No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGC80.22352612461581448No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG80.22352612461581448No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGC80.22352612461581448No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCT70.19558535903883767No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCC70.19558535903883767No Hit
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTT60.16764459346186086No Hit
ATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGA50.13970382788488406No Hit
TAGTCATCCCACTCGGCCTGGTAACACGTGCCAGCCACCGGGGCCCTGAGCTCATACTTTTTACGGAAGGACGCC50.13970382788488406No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGG50.13970382788488406No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGC50.13970382788488406No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA40.11176306230790724No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGA40.11176306230790724No Hit
GAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC40.11176306230790724No Hit
GCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACT40.11176306230790724No Hit
TTGACATTCTGATCAAATCCATCCATCTGATTCAGCAGCTCCAGCAGGATCCTCTGAACCTCCCTGTCGGCCCCT40.11176306230790724No Hit
GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCA40.11176306230790724No Hit
GTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTA40.11176306230790724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG305.495478E-446.00000420
ACCGTAC305.495478E-446.00000417
CCGTACT305.495478E-446.00000418
GACCGTA305.495478E-446.00000416
CGTACTA305.495478E-446.00000419
CTAGATC305.495478E-446.00000423
TCTCCGA305.495478E-446.0000041
ACTAGAT305.495478E-446.00000422
AGACCGT305.495478E-446.00000415
TAGATCT305.495478E-446.00000424
TACTAGA305.495478E-446.00000421
GAGACCG305.495478E-446.00000414
GATCTCG350.001174541839.42857426
TGCCGTC400.002264425234.535
CCGTCTT400.002264425234.537
TCTCGTA400.002264425234.528
TGCTTGA400.002264425234.545
ATGCCGT400.002264425234.534
GCTTGAA400.002264425234.546
CTGCTTG400.002264425234.544
Produced by FastQC (version 0.11.5)