FastQCFastQC Report
Wed 25 May 2016
SRR1033264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787753
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107970.3873011705125956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.037.5469671
GTATCAA70850.028.9599041
ATCAACG92600.023.1101063
TCAACGC92850.023.0046044
CAACGCA94850.022.3382135
AACGCAG96550.022.0885896
TATCAAC93450.021.9538232
GTGGTAT20000.021.3875031
ACGCAGA112000.018.8279197
GTACATG79650.018.774471
AATACCG2053.963396E-818.4562665
CGCAGAG114050.018.4295028
TGGTATC21450.018.1563362
TACATGG80150.018.004542
AGAGTAC112400.017.4164911
ACATGGG84400.017.406363
GAGTACT72150.017.26183512
CAGAGTA118800.016.8257510
GCAGAGT122600.016.6690439
AGTACTT75400.016.56252313
Produced by FastQC (version 0.11.5)