FastQCFastQC Report
Wed 25 May 2016
SRR1033264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787753
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.039.847391
GTATCAA77850.030.2235411
ATCAACG93550.024.9155733
TCAACGC94000.024.7962954
CAACGCA96600.024.12895
TATCAAC98400.023.8276822
AACGCAG98900.023.6026276
TACACCG2301.4551915E-1120.9878585
GTGGTAT19650.020.9238341
ACGCAGA115250.020.104647
TGGTATC19400.019.9060122
CGCAGAG116400.019.8171448
GAGTACT74050.019.41720612
AGAGTAC114900.019.17593811
AGTACTT76450.018.8076413
GTATACG1702.4008768E-618.2915461
CAGAGTA122600.018.2528210
GCAGAGT125800.018.1448369
GTACTTT78950.018.08106414
GTACATG83250.017.5139881
Produced by FastQC (version 0.11.5)