FastQCFastQC Report
Wed 25 May 2016
SRR1033263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063457
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41110.3865694616707587No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA29420.2766449419205478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.10343624612936865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.10277801547218177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG200.00743994451.60678511
CGCGAAC400.002592931734.50896561
GTACCGC751.6564463E-632.231343
TACGGGT450.004689952430.5760124
TACCGCC951.0423877E-525.4469744
ACTGCGG1101.1476459E-625.10338266
CGTACTT1103.3311226E-521.89896420
TATACTG2101.05501385E-1021.294015
TGAATCG1056.143832E-419.656015
TAAGCGC1108.187706E-418.83468628
TTAGGAC1108.396333E-418.7643263
GAGTACT50300.018.6728312
AGTACCG1301.1729971E-418.59321642
AGTACTT51450.018.32146313
GGTCATA950.00749434318.2980121
GTCGGGT950.007788138518.17640747
TGCGGTC950.007796669418.17295630
GTAGCTA950.007809479718.16778438
ACTCGTC950.0078180318.16433531
TTAGGCG950.007820168518.16347556
Produced by FastQC (version 0.11.5)