FastQCFastQC Report
Wed 25 May 2016
SRR1033263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063457
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17680.16625025741520344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.10089735645164778No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGC306.3282845E-445.9748332
GCCGGTT350.001351091439.40884828
CGTTATG350.001351403839.40699840
CGACGCG450.00457553330.73079955
TATACGT450.0046349330.6498874
GCCGTAG500.007686195427.6355721
ACGACGC658.6819916E-426.52518854
GGTCGAT1359.480573E-925.5415722
GTCGATC1452.0252628E-823.78120223
TATGCCG8950.023.50110243
ACCGTCG750.001999277222.9874158
AAGCCCG953.0757967E-421.77857628
GACTCGT800.002862786921.6096360
CCGTCGT800.002908512321.5507019
CTCGTAT9650.021.43904139
ACTTAGG1304.942616E-621.2201529
TATCTCG9450.021.16301736
TCTCGTA9650.021.08172438
GTATTAG1356.7469464E-620.4707931
ATCTCGT9950.020.44609337
Produced by FastQC (version 0.11.5)