FastQCFastQC Report
Wed 25 May 2016
SRR1033262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454908
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44740.3075108529199097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.031.3143251
GTATCAA45250.028.2078531
ATCAACG54900.023.6928123
TCAACGC55200.023.3746094
CAACGCA56000.022.9792445
AACGCAG56900.022.7971866
TATCAAC56550.022.5074142
GTATAGG4000.021.7371481
GAGTACT40350.020.63735212
AGAGTAC60850.019.84854511
ACGCAGA64900.019.8280097
CGCAGAG64700.019.782948
AGTACTT42300.019.6039613
GGACCGC1258.925296E-519.2681586
GTGGTAT14450.019.2550531
GTACTTT43650.018.76375814
CCGATAA950.00795456318.110429
GCAGAGT69150.018.063289
CAGAGTA68700.017.93116210
GTACATG40000.017.7375131
Produced by FastQC (version 0.11.5)