FastQCFastQC Report
Wed 25 May 2016
SRR1033262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454908
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.032.3402521
ATAACGC951.0543292E-525.4107463
GTATCAA52700.023.5311851
CCCGTCG953.07458E-421.7806439
CGGACTA1108.258741E-418.811224
ATCAACG65350.018.7865643
TCAACGC65950.018.6156484
GTGGTAT18750.018.422931
TATCAAC67100.018.2966022
CAACGCA67650.018.147855
AACGCAG69650.017.7257616
TGGTATC20000.016.8981462
GAGTACT49000.016.53977812
TACACTG8550.016.5371515
AGTACTT49950.016.36329713
GTCACGG1907.0463666E-616.3360424
ACGTTCA1704.9795948E-516.22871239
TATACTG6200.016.1305545
AGAGTAC71000.015.83496811
GTACTTT52650.015.65515714
Produced by FastQC (version 0.11.5)