FastQCFastQC Report
Wed 25 May 2016
SRR1033261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103115
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121650.3920254325089466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.039.367291
GTATCAA81800.029.4615021
ATCAACG104950.023.4700553
TCAACGC106100.023.212664
CAACGCA107650.022.8145265
TATCAAC105100.022.7304272
GTGGTAT19350.022.6445921
AACGCAG111200.022.2109956
ACCGTCC3650.021.6772638
TAGATCG1154.7126334E-520.9376285
TGGTATC22000.019.2837182
GTACATG92650.019.1425461
ACGCAGA132650.018.5165657
CGCAGAG131950.018.5105138
CGACCGT1501.6782504E-518.42176421
TACATGG94150.018.3564912
TGAACCG4550.018.1437515
ACATGGG97350.017.8105833
GAGTACT87150.017.7243212
GTCTAGC3600.017.3878121
Produced by FastQC (version 0.11.5)