FastQCFastQC Report
Wed 25 May 2016
SRR1033261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103115
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.039.081681
GTATCAA87150.031.4064391
ATCAACG108550.024.8077453
TCAACGC109450.024.6352564
TATCAAC111350.024.2458632
CAACGCA111750.024.0665135
AACGCAG115050.023.4960866
GTCGTAC1206.308122E-520.1594051
GAGTACT92150.019.75625812
GTGGTAT21350.019.747991
ACGCGAC1056.0498464E-419.70280324
ACGCAGA137800.019.4919077
CGCAGAG138100.019.3996288
AGTACTT94450.019.05612613
GTACATG92350.018.860561
GTACTTT98800.018.25201614
AGAGTAC139000.018.1329611
TACATGG94450.018.106972
TGGTATC22800.017.9960922
GCAGAGT147550.017.7598979
Produced by FastQC (version 0.11.5)