FastQCFastQC Report
Wed 25 May 2016
SRR1033260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097868
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81410.3880606406122788No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG28390.13532786619558523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA500.00785016727.51750814
GCGCGTT500.00786303227.50827413
GTCGTGC700.001360151924.5615488
TACGCGA750.002026585922.93510216
CGCCTAC1103.3523756E-521.8826312
CGAACGC800.00288071621.5875844
AATCGCA850.00409535120.31625649
CGGATAC850.0041136520.3006259
AGTAGCG850.0041136520.3006260
GCGTAAC850.00420032520.2276411
TTACGCG900.005831967619.1116715
TACCTCG1451.2968254E-518.98077416
TCGAGGG1301.1787115E-418.58287461
GTACATG122050.018.5384271
GCGAACG950.00778417918.17901643
ACGAACG950.007789582518.17682852
CGAACGG950.007803646418.17114368
TCGAACG1150.001105033918.01356567
ACATGGG121250.017.9796333
TACATGG124650.017.955262
Produced by FastQC (version 0.11.5)