FastQCFastQC Report
Wed 25 May 2016
SRR1033260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097868
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA130440.6217741059018013No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG32000.15253581254873996No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25970.12379234537158677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTA400.002603733434.4813047
AGGTACG605.3527486E-428.81477257
CGTTGCG605.440937E-428.7344236
GTCACGC605.440937E-428.7344249
CGTCACG658.686944E-426.52407848
GGTTCGC951.0557364E-525.40727636
GCCGTAA700.001338434624.62950134
AGTCGTC851.4491747E-424.33974353
CGAAATA1452.028537E-823.78020939
GCGCGCA1452.028537E-823.78020949
ATCTCGT8850.023.76677337
CTCGTAT9200.023.61219639
TATGCCG9250.022.73902343
TCTCGTA9300.022.6167738
CATACCG1004.3523387E-420.68878231
TCGTATG10250.020.5205840
CGTTAGT850.00412912220.28747262
CGTCAGA1701.0922122E-720.283122
CGTATGC10550.019.93705641
TTGAGCG1601.3700119E-619.39573324
Produced by FastQC (version 0.11.5)