FastQCFastQC Report
Wed 25 May 2016
SRR1033259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287155
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89230.39013534281673085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.040.4469761
GTATCAA65150.029.2035271
ATCAACG80750.023.2107333
TCAACGC81850.022.8942684
CAACGCA83650.022.360525
TATCAAC83250.022.2677522
GTGGTAT16450.021.9902341
AACGCAG86300.021.9547216
TGGTATC17050.020.806992
ACGCAGA98800.019.1087047
CGCAGAG99950.018.751228
GAGTACT67900.017.77912912
CTGTCGC4650.017.751359
AGAGTAC99600.017.57646611
AGTACTT70350.017.25621413
GCAGAGT106450.017.1562359
CAGAGTA105650.016.99315810
GTACATG64600.016.422141
TGTCGCC5250.016.37773310
TCTGTCG4500.016.0480638
Produced by FastQC (version 0.11.5)