FastQCFastQC Report
Wed 25 May 2016
SRR1033259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287155
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.040.3492241
GTATCAA70550.028.4569261
GTGGTAT17650.025.4511571
ATAACGC1452.0299922E-823.7788263
TATAACG1352.6770977E-722.9861982
ATCAACG87100.022.8014643
TCAACGC87450.022.7102054
TATCAAC88850.022.5463942
TGGTATC19400.022.393772
CAACGCA89200.022.3033125
AACGCAG91550.021.6931466
ACGCAGA105800.018.673577
CGCAGAG107450.018.354738
AGAGTAC106900.017.35253711
GAGTACT73000.016.90902512
CAGAGTA112650.016.89531510
GCAGAGT115550.016.769689
GTCTAGG4450.016.3067781
AGTACTT75750.016.20413213
TCGCCGA1300.002532859315.91352217
Produced by FastQC (version 0.11.5)