FastQCFastQC Report
Wed 25 May 2016
SRR1033258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629451
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63410.389149474270782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30950.033.0215031
GTATCAA49850.025.9410721
ATACCGT1554.2306056E-822.1866726
ATCAACG61900.021.1139953
TCAACGC61800.020.9779664
CAACGCA62950.020.6493595
TATCAAC62900.020.1696412
AACGCAG64750.020.1290536
CGAATTA1056.1357836E-419.66075315
GTACATG46350.018.7499641
TACATGG45900.018.4013462
GAGTACT42950.018.25557712
ACCGTCC950.00798085218.1002168
ATCGCTA1351.5692382E-417.90107367
ACGCAGA73050.017.8425247
CGCAGAG72800.017.8093198
AGAGTAC66250.017.59696211
ACATGGG49050.017.1792643
AGTACTT45650.017.10050613
ACACCGT1653.9504506E-516.6736226
Produced by FastQC (version 0.11.5)