FastQCFastQC Report
Wed 25 May 2016
SRR1033258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629451
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.036.2157631
GTATCAA51450.027.401811
ATCAACG63350.021.8814853
TCAACGC63650.021.8325234
CAACGCA65300.021.3336665
TATCAAC65500.021.1632372
AACGCAG67300.020.6490756
ACCGCGT850.004132377420.284388
GTACATG47050.018.6542841
GGACCGC1852.6580892E-718.63976
ACGCAGA75350.018.3057447
CGCAGAG75500.018.1780288
TACATGG47150.017.9176772
CTGACGT1351.5836264E-417.8797554
AGAGTAC71500.017.69989411
ACACCGT2551.376975E-917.5797946
GAGTACT49750.017.32836512
GTGTAAG4200.017.2772431
AGTACTT50000.017.10326413
ACATGGG48200.016.8834933
Produced by FastQC (version 0.11.5)