FastQCFastQC Report
Wed 25 May 2016
SRR1033257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884912
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN115420.40008152761678695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.040.262061
GTATCAA77250.029.7141461
GTGGTAT17800.028.3312551
TGGTATC19600.025.189972
ATCAACG94400.024.7078783
TCAACGC96250.024.230444
CAACGCA97950.023.8099045
TATCAAC96550.023.6956732
AACGCAG102150.022.899086
ACGCAGA115650.019.8995447
CGCAGAG116450.019.6446728
CCGAACG900.005712647519.18047150
GAGTACT78250.019.16813712
AGTACTT79850.018.56831613
AGAGTAC115650.018.5319111
CAGAGTA119700.018.33598710
GTACATG77200.018.2905391
GCAGAGT123800.018.117779
GTACTTT84150.017.62409614
TACATGG78350.017.1738382
Produced by FastQC (version 0.11.5)