FastQCFastQC Report
Wed 25 May 2016
SRR1033257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884912
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.043.2478871
GTATCAA76100.027.8376391
TATAACG1752.4374458E-1023.6443672
GTGGTAT17500.022.7099591
ATCAACG93850.022.3017483
TCAACGC94800.022.0418874
TGGTATC17950.021.899032
TATCAAC97050.021.7085192
CAACGCA96200.021.6852685
AACGCAG98950.021.152296
CGCGTAT1150.001114679717.99059112
GTACATG83300.017.8393921
ACGCAGA116300.017.7298877
CGCAGAG117950.017.4818658
ATACCGT2251.1161137E-716.8575576
AGAGTAC118200.016.59918811
GTACCGT1250.001948592716.5510566
TACATGG84850.016.5396792
TACCGTC1907.062519E-616.333287
CAGAGTA123650.016.06248910
Produced by FastQC (version 0.11.5)