FastQCFastQC Report
Wed 25 May 2016
SRR1033256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897962
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN153650.3941803434717937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG751.7070106E-632.1111649
TATACCG2100.024.571075
GGTATCA112750.024.464151
TAGCGTA902.1689375E-422.9347727
GTATCAA169250.022.2778931
GTACCGA1103.3399425E-521.89397810
ACCGTCC2454.0017767E-1119.6583778
CGTTGCG900.005816530419.12094320
ATCAACG199600.019.1157053
TCAACGC200350.019.058124
AACGCAG201600.018.9236246
CAACGCA202850.018.789575
CGTGCGA1301.17433025E-418.59258744
GTACATG182250.018.417611
TATCAAC204500.018.2634372
TACATGG177650.018.2245162
CGCGGTA3103.6379788E-1217.82146643
ACATGGG180950.017.6444723
CGTAGCG1402.1231956E-417.20818315
GAGTACT104900.016.95642712
Produced by FastQC (version 0.11.5)