FastQCFastQC Report
Wed 25 May 2016
SRR1033256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897962
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113900.026.5354861
TATGCCG7800.023.8707943
CTCGTAT8100.022.98698439
ACGATAG750.002000595422.9866933
TTACGCG1052.2928287E-522.98668943
CGAACGA1103.28381E-521.94212216
GTATCAA168950.021.6920151
TACGCGA1206.420621E-520.11335444
TCTCGTA9350.019.91385838
GTACATG190900.018.799761
ATCTCGT10100.018.77649937
GCCGTCT10350.018.32272346
TACATGG185600.018.3175182
ATCAACG200700.018.0904223
TCAACGC201600.017.9412484
AACGCAG203000.017.8345016
CAACGCA203800.017.7644945
ACATGGG185350.017.6353233
TATCAAC207850.017.534472
TCGTATG11200.016.93237540
Produced by FastQC (version 0.11.5)