FastQCFastQC Report
Wed 25 May 2016
SRR1033255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834313
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111300.39268775184674376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.038.76561
GTATCAA80000.031.3409881
ATCAACG99650.025.6520463
TCAACGC100500.025.46394
CGACCGT1101.1440734E-625.11522521
CAACGCA102700.024.8853725
TATCAAC101300.024.3351552
AACGCAG106500.024.3208546
GTGGTAT19700.023.4775521
TGGTATC19550.022.408932
ACGCAGA121350.021.0906377
TCGAACG1154.5814726E-521.01436253
CGCAGAG122800.020.7295518
GAGTACT84300.020.12044312
AGAGTAC120250.019.6557611
AGTACTT88400.019.18759713
GCAGAGT128100.019.0958359
CAGAGTA128800.018.69822510
GTACTTT91900.018.27548814
GTACATG76800.017.8855921
Produced by FastQC (version 0.11.5)