FastQCFastQC Report
Wed 25 May 2016
SRR1033255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834313
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.039.0385861
GTGGTAT19850.029.2421361
GTATCAA80800.029.0775531
TGGTATC21950.025.1327952
ATCAACG103000.022.4281223
TCAACGC103100.022.306044
TATCAAC106000.021.9885272
CAACGCA105250.021.9159015
AACGCAG109650.021.0679116
TACACCG3000.020.6874315
CGCAGAG125150.018.238228
ACGCAGA125750.018.2060367
ACACCGT3650.017.9479986
GAGTACT86400.017.83874912
GTACATG81150.017.5415921
TCTATAC9800.017.2395273
AGAGTAC125400.017.18514311
TACATGG80650.016.8013232
GCAGAGT134950.016.6841189
CAGAGTA132050.016.65885710
Produced by FastQC (version 0.11.5)