FastQCFastQC Report
Wed 25 May 2016
SRR1033254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181034
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165760.3964569529929678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.038.3633461
GTATCAA109150.029.0202621
ATCAACG138900.023.3245533
TCAACGC138950.023.2635784
CAACGCA142800.022.564135
TATCAAC141600.022.0724372
AACGCAG150050.021.566866
GTGGTAT29800.020.7687511
TGGTATC31650.019.003182
ACGCAGA170100.018.7828037
CGCAGAG170100.018.6210588
GAGTACT102350.017.40672312
GTACATG126500.017.3163621
AGAGTAC166800.017.2173211
AGTACTT104150.016.90754513
CAGAGTA177500.016.64446810
GCAGAGT183100.016.529879
TACATGG126500.016.3523562
GTACTTT109950.015.9571214
TATACCG2705.4726115E-815.2835655
Produced by FastQC (version 0.11.5)