FastQCFastQC Report
Wed 25 May 2016
SRR1033254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181034
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62050.038.5798231
GTATCAA108150.029.576941
GTGGTAT28850.024.306311
ATCAACG135750.023.1642383
TCAACGC136550.023.0537784
CAACGCA140200.022.4781845
TATCAAC142150.022.4123882
AACGCAG145300.021.7369236
TGGTATC29850.021.7158362
CGAACTA3650.018.8931929
ACGCAGA170050.018.4718327
CGCAGAG170950.018.2333958
GAGTACT104600.017.24003812
AGAGTAC170200.017.09822811
GTACATG126450.016.93721
CAGAGTA180400.016.53285210
AGTACTT108900.016.46411913
GCAGAGT185550.016.4270489
ATCGTAC2101.0050626E-616.41908325
TATACCG2351.8328683E-716.1394185
Produced by FastQC (version 0.11.5)