FastQCFastQC Report
Wed 25 May 2016
SRR1033253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468238
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135200.3898233050903658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53500.037.5906031
GTATCAA89150.028.6470491
ATCAACG117100.022.3268663
TCAACGC117150.022.3141044
CAACGCA119800.021.820515
TATCAAC117750.021.596912
AACGCAG122550.021.4995776
GTGGTAT25050.019.029091
GTACATG109650.018.8805031
TATACCG2204.3564796E-918.7614655
ACGCAGA139900.018.612527
CGCAGAG141700.018.4003648
CGAACTA3450.018.01470629
TACATGG110100.017.9155792
AGAGTAC137200.017.65029311
TACACCG4550.017.3869955
GAGTACT83200.017.28169312
TGGTATC26600.017.132662
ACATGGG114300.017.125263
CAGAGTA144850.017.00333410
Produced by FastQC (version 0.11.5)