FastQCFastQC Report
Wed 25 May 2016
SRR1033253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468238
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54450.038.5164031
GTATCAA89550.027.3163471
ATCAACG112850.021.4492953
TCAACGC113850.021.3214684
CAACGCA115450.021.085715
GTGGTAT24550.020.6881071
TATCAAC118300.020.5777262
AACGCAG120350.020.2845156
GTACATG117450.019.5624621
TACATGG119300.018.2953512
TGGTATC26700.017.9506842
GTACTAG4150.017.483411
ACGCAGA140050.017.2342517
TATACTG16000.017.0249025
CGCAGAG142650.016.895968
ACATGGG122900.016.8897063
GAGTACT82100.016.4636712
AATACCG2955.4023985E-1016.3637755
ACACCGT5700.016.3330156
AGAGTAC138500.016.30704511
Produced by FastQC (version 0.11.5)