FastQCFastQC Report
Wed 25 May 2016
SRR1033252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879384
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73670.3919901414506029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCC450.00467423330.59821915
ACGACCG700.001330270524.65519769
GGTATCA84000.022.275571
TCGCCCA2351.8189894E-1221.96448112
TACGGCT800.00294662721.5034354
GGGCGTA2051.7298589E-920.20632451
GTATCAA117150.019.9207671
CGTAATG2153.1341187E-919.26442354
GTCCTAG5500.018.3383871
TCGATAA950.00778947818.1767522
GAGTACT70300.018.11106512
GTACATG94100.017.8518541
TACATGG93150.017.84532
CGAAGTA1200.001470180817.26234639
ACATGGG98250.017.2331623
CTACGGC1402.1236707E-417.2068923
AACGCAG138700.017.017166
AGTACTT76350.016.81069813
ACTTACC4100.016.7840678
ATCAACG140950.016.7735163
Produced by FastQC (version 0.11.5)