FastQCFastQC Report
Wed 25 May 2016
SRR1033252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879384
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA756.18693E-527.58598311
TATGCCG9500.024.6815443
GCGTTAC851.4490218E-424.33992819
GGTATCA85700.023.4996321
GACCGAT1052.2912252E-522.9877097
CCGATCG902.1347677E-422.9877079
ACCGATC1854.8748916E-1022.3664198
CTCGTAT10500.021.67470239
CGTATGC11300.021.36082841
CTGTCGC3400.021.2974369
GTATGCC11200.020.93523642
TCGAATC1004.3511033E-420.68948716
TCGTATG11600.020.51113140
TCGCCCA2402.910383E-1120.11477912
ATGCCGT11500.020.08925844
GTATCAA118500.019.9685061
GCAATCG1056.0455874E-419.70427512
TGCCGTC11600.019.61882245
ACCGCGT900.005753486419.1564248
GGCGTTA900.005753486419.15642442
Produced by FastQC (version 0.11.5)