FastQCFastQC Report
Wed 25 May 2016
SRR1033251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726220
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145820.39133491849649243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.040.535221
GTATCAA88200.029.5219021
ATCAACG111600.023.665463
TCAACGC111050.023.6243294
CAACGCA113700.023.1039585
TATCAAC114200.022.4928362
AACGCAG118650.022.2288116
GTGGTAT23800.020.8876761
ACGCAGA136050.019.2352647
CGCAGAG136650.019.1004778
TGGTATC24300.019.0244142
GTACATG101250.018.5408571
GAGTACT92700.017.69620112
AGAGTAC136950.017.50298511
TACATGG102450.017.3370362
ACATGGG107050.016.993663
GCAGAGT146600.016.9842349
CAGAGTA143800.016.98012410
AGTACTT96250.016.82889713
GTACTTT99500.016.3190614
Produced by FastQC (version 0.11.5)