FastQCFastQC Report
Wed 25 May 2016
SRR1033251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726220
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.039.6404841
GTATCAA91500.029.1853141
ATCAACG113900.023.2193093
TCAACGC114300.023.1078834
CAACGCA118300.022.3848465
TATCAAC120150.022.154972
GTGGTAT24000.021.8795451
AACGCAG122600.021.712236
TGGTATC25700.019.1858272
ACGCAGA141750.018.6816757
CGCAGAG142550.018.5526438
AATACCG4300.018.4432335
GAGTACT96200.018.35154212
CGAACTA3200.018.31818429
GTACATG103600.017.8735561
TACATGG101150.017.5557352
AGTACTT99700.017.49979613
AGAGTAC143250.017.37881711
TCCGATA1402.0930289E-417.24041445
GTACTTT102850.016.89677814
Produced by FastQC (version 0.11.5)