FastQCFastQC Report
Wed 25 May 2016
SRR1033249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210449
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166520.3954922622266651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75600.038.077231
GTATCAA142250.027.1286071
GCTCTAG9600.024.6260321
CTAGACG851.4755204E-424.2760284
ATCAACG173650.022.6018643
TCAACGC177000.022.11134
CAACGCA178100.021.9940435
GCGAACT16400.021.89781628
CCGAAAT16100.021.66422847
AACGCAG182500.021.4451876
TCGCCCC17350.020.6950838
GTACATG190450.020.5000821
TATGCGC850.004112437420.30223558
TATCAAC192150.020.0446532
AAAGCGA17800.019.98726825
TACATGG178000.019.8023662
CGCACGA1055.990087E-419.73209828
CGGTAGA7050.019.52196514
CAATACG4950.019.46466817
AACCGGC7300.019.399823
Produced by FastQC (version 0.11.5)